Extraction Application
FUST Lab: The company changing
the world through technology
the world through technology
Non-destructive
Organic Solvent-free Extraction
Non-destructive Organelle Extraction
Precise Extraction of Cellular Organelles
Extracting mitochondria from cells enables mitochondrial-based drug development
across various pharmaceutical industries. Given mitochondria's role in metabolic disorders
(Parkinson's disease, Alzheimer's disease), this facilitates diverse research and development.
Existing Technology
Centrifuge and homogenizer-based extraction
Low efficiency, high processing time
Organelle destruction
Uneven extraction
DEBREX
Focused Ultrasonic-based Extraction
Extracts without organelle damage
High efficiency achieved through continuous
processing
Uniform extraction with ultrasonication
Confirmation of cell disruption via ultrasonic treatment
Before
After
Confirmation of no cell movement
Ultrasonic power control
Disruption rate adjustable via power control
Mitochondria Extraction Study in Human Hepatic Cells (WRL-68)
Purpose & Background
Verification of a mitochondria extraction method using human hepatic cells (WRL-68) for liver disease and metabolic research, disease diagnosis/therapeutics (mitochondrial dysfunction, biomarkers), and biotech product development
Mitochondria are essential organelles for ATP (cellular energy) production. Extracted mitochondria can be utilized in studies related to myocardial infarction, Parkinson’s disease, Alzheimer’s disease, and various metabolic disorders
Applicable for early diagnosis of cancer/metabolic diseases and development of therapeutic candidates
Key Technologies for Mitochondria Extraction
It is essential to extract mitochondria efficiently without damaging their structure (mtDNA and membrane potential), as hepatic cells are highly delicate and prone to loss during the process
As hepatic cells are highly delicate, maintaining extraction efficiency and structural integrity is critical for technology scalability and reproducibility
Mitochondria Extraction Results Using Focused Ultrasonic Technology
Cell Line : WRL-68 (Human hepatic cells)
Mitochondrial Structural Stability Analysis
Structural integrity was preserved with no observable morphological damage
Comparative staining results confirmed that the outer mitochondrial membrane remained preserved at approximately 99% integrity
Mitochondrial Membrane Potential Analysis
Verified the normal maintenance and preservation of mitochondrial membrane potential
TMRE staining analysis showed that the mitochondrial membrane potential remained stable compared to the control (TMRE: 99%)
Organic Solvent-free Extraction
High-content extraction without organic solvents
Conventional active ingredient extraction processes relied on ethanol, organic solvents, and
high-temperature heating, leading to various issues such as skin irritation from toxic
byproducts and denaturation of active components.
DEBREX is an ultrasonic extraction device capable of water-based extraction,
enabling stable extraction of high-content, high-purity active ingredients.
Extract from sprouted oats
Solvent: Water 100%
Temperature : 60°C
Amaranth extraction
Solvent: Water 100%
Temperature : Room temperature 25°C
Extraction time drastically reduced (96% reduction)
Performance improved by 1.69 times
| Solvent | Time | Antioxidant performance | |
|---|---|---|---|
| Conventional method | 70% Ethanol + 30% Water | 1440minutes (24hours) | 0.55 |
| DEBREX | 100% Water | 60minutes (1hour) | 0.96 |
